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human pulmonary microvascular ecs hpmec  (PromoCell)


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    PromoCell human pulmonary microvascular ecs hpmec
    Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal <t>HPMEC</t> compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.
    Human Pulmonary Microvascular Ecs Hpmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+microvascular+ecs+hpmecs/HPMEC-c+Human+Pulmonary+Microvascular+Endothelial+Cells/pmc12668437-30-10-22
    Average 96 stars, based on 163 article reviews
    human pulmonary microvascular ecs hpmec - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Acute endothelial stresses identify microRNA let-7b-5p and non-coding SLC11A2 (NRAMP2/DMT1) exon as biomarkers that overlap with those detected in malignant and non-malignant diseases"

    Article Title: Acute endothelial stresses identify microRNA let-7b-5p and non-coding SLC11A2 (NRAMP2/DMT1) exon as biomarkers that overlap with those detected in malignant and non-malignant diseases

    Journal: QJM: An International Journal of Medicine

    doi: 10.1093/qjmed/hcae235

    Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal HPMEC compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.
    Figure Legend Snippet: Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal HPMEC compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.

    Techniques Used: RNA Sequencing, Binding Assay, Quantitative RT-PCR

    Related Articles

    Cell Culture:

    Article Title: NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia
    Article Snippet: .. Human pulmonary microvascular ECs (HPMECs) (Promocell, Heidelberg, Germany) and human pulmonary artery ECs (HPAECs) (Lonza, Walkersville, MD) were cultured in EC growth medium (MV-2, Promocell) containing EC growth medium supplement mix (Promocell) as described ( 32 ). ..

    Article Title: LPS binding to HMGB1 promotes angiogenic behavior of endothelial cells through inhibition of p120 and CD31 via ERK/P38/Src signaling.
    Article Snippet: Lipopolysaccharide (LPS) is known to mediate angiogenic effects in endothelial cells.. The underlying mechanisms, however, remain largely unknown.. In this study, we showed that LPS induced high motility group box protein 1 (HMGB1) secretion in human pulmonary microvascular endothelial cells (HPMECs).



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    Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal <t>HPMEC</t> compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.
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    Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal <t>HPMEC</t> compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.
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    Loss of ERG and FLI1 induces the expression of inflammatory and IFN genes in <t>HPMECs.</t> Human pulmonary <t>microvascular</t> ECs (HPMECs) were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. Scrambled siRNA (siRNA) was used as a control. Relative mRNA expression was determined by quantitative RT-PCR (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.
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    Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal HPMEC compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.

    Journal: QJM: An International Journal of Medicine

    Article Title: Acute endothelial stresses identify microRNA let-7b-5p and non-coding SLC11A2 (NRAMP2/DMT1) exon as biomarkers that overlap with those detected in malignant and non-malignant diseases

    doi: 10.1093/qjmed/hcae235

    Figure Lengend Snippet: Pre-miRNA and mature miR responses to ferric citrate treatment of normal primary ECs. ( A ) RNA-seq data for all pre-miRNAs detected in 1 and 6 h rRNA-depleted libraries from EC treated with media or 10 μmol/l ferric citrate, presented as fold change of respective media-treated EC represented at 0 h. One hour data are from normal HDMEC compared to paired 1 h media-treated HDMEC. Six hour data are from normal HPMEC compared to paired 6 h media-treated HPMEC. By Dunn’s test post Kruskal–Wallis, there was a fall (* P < 0.05) after 1 h treatment, and no significant change after 6 h. ( B ) RNA-seq data for let-7 pre-miRNAs as in (A) (let-7a-1, let-7a-2, let-7a-3, let-7b, let-7d, let-7f-1, let-7g, let-7i), with P values (* P < 0.05) calculated by Dunn’s test post Friedman. ( C ) RNA-seq data for mRNA changes in HPMEC after 6 h 10μmol/l ferric citrate, as fold change of respective values in paired media-treated HPMEC, categorized by whether mRNAs were identified by TargetScan 5.2[28] as a let-7 target based on mature miRNAs from miRbase: 570 of 10 851 mRNAs had 1-6 (mean 1.11) let-7 family 8mer, 7mer-m8 or 7mer-1A binding sites . ( D ) Comparisons of let-7b by qRT-PCR and RNA-seq. (i) Mature miRNA let-7b-5p, assayed at stated durations of 10μmol/l ferric citrate as quantified in HPMEC by qRT-PCR using Applied Biosystems miRNA assay 002619. Ct values were converted to concentrations following spiking of cell extracts with known concentrations of cel-miR-39 as described in the . (ii) RNA-seq comparisons (as also represented in A and B). ( E ) Dose–response curves for mature let-7b-5p in four cultures of primary HDMEC or primary HUVEC, after 1 h treatment with media (‘0’), 4 or 10 μmol/l ferric citrate treatments, quantified by qRT-PCR using miRNA assay 002619 (Applied Biosystems). Threshold cycles (Ct) values were converted to concentrations based on known concentrations of spiked cel-miR-39 (described further in ), before fold-changes from the media-treated EC were calculated for graphical presentation.

    Article Snippet: For treatment duration, no morphological changes were observed in primary human pulmonary microvascular ECs (HPMEC) or primary human dermal microvascular ECs (HDMEC, PromoCell GmbH, Heidelberg) cultured for 24 h with 10 μmol/l ferric citrate: 1 h and 6 h responses were evaluated.

    Techniques: RNA Sequencing, Binding Assay, Quantitative RT-PCR

    Loss of ERG and FLI1 induces the expression of inflammatory and IFN genes in HPMECs. Human pulmonary microvascular ECs (HPMECs) were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. Scrambled siRNA (siRNA) was used as a control. Relative mRNA expression was determined by quantitative RT-PCR (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Synergistic Role of Endothelial ERG and FLI1 in Mediating Pulmonary Vascular Homeostasis

    doi: 10.1165/rcmb.2016-0200OC

    Figure Lengend Snippet: Loss of ERG and FLI1 induces the expression of inflammatory and IFN genes in HPMECs. Human pulmonary microvascular ECs (HPMECs) were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. Scrambled siRNA (siRNA) was used as a control. Relative mRNA expression was determined by quantitative RT-PCR (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.

    Article Snippet: Human pulmonary artery ECs (HPAECs) and human pulmonary microvascular ECs (HPMECs) were purchased from Lonza (Basel, Switzerland).

    Techniques: Expressing, Control, Quantitative RT-PCR, Comparison

    Loss of ERG and FLI1 induces the expression of IRF proteins in HPMECs. HPMECs were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. siSCR was used as a control. Representative immunoblots and bar graphs with the densitometric analysis are shown (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Synergistic Role of Endothelial ERG and FLI1 in Mediating Pulmonary Vascular Homeostasis

    doi: 10.1165/rcmb.2016-0200OC

    Figure Lengend Snippet: Loss of ERG and FLI1 induces the expression of IRF proteins in HPMECs. HPMECs were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. siSCR was used as a control. Representative immunoblots and bar graphs with the densitometric analysis are shown (n = 3 independent experiments). Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. *P < 0.05, **P < 0.01.

    Article Snippet: Human pulmonary artery ECs (HPAECs) and human pulmonary microvascular ECs (HPMECs) were purchased from Lonza (Basel, Switzerland).

    Techniques: Expressing, Control, Western Blot, Comparison

    Down-regulation of ERG and FLI1 in HPMECs induces increased cell monolayer permeability. HPMECs were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. siSCR was used as a control. Fluorescence of 3 kD dextran sulfate–FITC that penetrated the endothelial layer grown on transwell inserts was measured. Vascular endothelial growth factor (VEGF; 100 ng/ml) treatment was used as a positive control. The experiment was done in triplicate. Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. **P < 0.01, ***P < 0.001. AU, arbitrary units; MFI, mean fluorescent intensity.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Synergistic Role of Endothelial ERG and FLI1 in Mediating Pulmonary Vascular Homeostasis

    doi: 10.1165/rcmb.2016-0200OC

    Figure Lengend Snippet: Down-regulation of ERG and FLI1 in HPMECs induces increased cell monolayer permeability. HPMECs were subjected to siRNA-mediated silencing of FLI1 and/or ERG for 48 hours. siSCR was used as a control. Fluorescence of 3 kD dextran sulfate–FITC that penetrated the endothelial layer grown on transwell inserts was measured. Vascular endothelial growth factor (VEGF; 100 ng/ml) treatment was used as a positive control. The experiment was done in triplicate. Data shown as mean (±SD). One-way ANOVA followed by Tukey’s multiple comparison were used for statistical analysis. **P < 0.01, ***P < 0.001. AU, arbitrary units; MFI, mean fluorescent intensity.

    Article Snippet: Human pulmonary artery ECs (HPAECs) and human pulmonary microvascular ECs (HPMECs) were purchased from Lonza (Basel, Switzerland).

    Techniques: Permeability, Control, Fluorescence, Positive Control, Comparison